Effect of propofol on pyroptosis of lung cancer A549 cells by NLRP3/ASC/caspase-1 pathway
10.12007/j.issn.0258-4646.2024.02.007
- VernacularTitle:丙泊酚作用NLRP3/ASC/caspase-1通路对肺癌A549细胞焦亡的影响
- Author:
Jichi GUAN
1
;
Jinyu YANG
;
Dan LIU
;
Lei LI
;
Donghua LI
;
Haifeng WANG
Author Information
1. 沈阳化工大学制药与生物工程学院,沈阳 110142
- Keywords:
propofol;
ultra-low attachment plates;
three-dimensional culture model of tumor cells;
lung cancer;
pyroptosis
- From:
Journal of China Medical University
2024;53(2):132-135,141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of propofol on pyroptosis and A549 cells via the NLRP3/ASC/caspase-1 pathway.Methods Establish a three-dimensional culture model of A549 tumor cells using ultra-low attachment plates,A549 cells were cultured using ultra-low adsorption culture plates to establish a three-dimensional culture model.The CCK-8 method was used to detect the effect of propofol on A549 cell proliferation;the inflammatory factors interleukin(IL)-18,IL-1β,and IL-6 were detected in the A549 lung cancer cell supernatants using enzyme-linked immunosorbent assays;western blotting was used to detect the expression levels of pyrolysis-asso-ciated proteins NLRP3,ASC,caspase-1,GSDMD-N,and IL-1β in A549 lung cancer cells in each group.Results Compared to the blank control group,the survival rate of A549 cells in low,medium,and high concentrations of propofol in each group decreased in turn(P＜0.05);the levels of inflammatory factors IL-18,IL-1β,and IL-6 in the A549 cell supernatant,and scorch related protein NLRP3,ASC,caspase-1,GSDMD-N,and IL-1β increased with the increased propofol concentrations(P＜0.05).Conclusion The three-dimensional culture model of lung cancer A549 cells was successfully established using the ultra-low adsorption culture method.Propofol can promote cell apoptosis and inhibit the pyroptosis of A549 lung cancer cells via activating the NLRP3/ASC/caspase-1 pathway.
