High salt diet leads to abnormal mitochondrial function in mouse ovarian tissue
10.12007/j.issn.0258-4646.2024.02.003
- VernacularTitle:高盐饮食导致小鼠卵巢组织线粒体功能异常
- Author:
Entai HOU
1
,
2
,
3
,
4
;
Xiaoyang GU
;
Shifeng NI
;
Xinyu LI
;
Jie QIAO
Author Information
1. 北京大学第三医院妇产科生殖医学中心,北京 100191
2. 国家妇产科疾病临床医学研究中心 (北京大学第三医院),北京 100191
3. 辅助生殖教育部重点实验室 (北京大学),北京 100191
4. 北京市生殖内分泌与辅助生殖技术重点实验室,北京 100191
- Keywords:
high salt diet;
mouse ovary;
mitochondrial dysfunction;
electronic transfer chain;
succinate dehydrogenase
- From:
Journal of China Medical University
2024;53(2):108-113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effect of a high salt diet on ovarian mitochondrial function.Methods Twenty female ICR mice were randomly divided into a normal salt diet(NSD)group and a high salt diet(HSD)group(n = 10 each).The NSD group was given a normal salt diet and the HSD group was given an 8%NaCl diet for 4 weeks.A high salt-treated cell model was established by inducing COV-434 cells cultured in vitro with NaCl.Western blotting was used to detect the protein expression of superoxide dismutase(SOD)and ComplexesⅠ-Ⅴ.The activity of SOD and succinate dehydrogenase(SDH)was detected kinetically.A chemiluminescence assay was used to detect adenosine triphosphate(ATP)levels.Results Compared with the NSD,the HSD significantly reduced the expression level of ComplexⅠin ovarian mitochondria(P＜0.01),significantly increased the expression level of ComplexⅤ(P＜0.05),and significantly reduced the activity of SDH and content of ATP(P＜0.01).The expression level of ComplexesⅠandⅡdecreased significantly(P＜0.05),expression level of ComplexⅤ increased significantly(P＜0.05),activity of SDH decreased significantly(P＜0.01),and content of ATP was insufficient(P＜0.01)in COV-434 cells cultured under high salt conditions.Conclusion High salt can lead to mitochondrial dys-function in the mouse ovary,such as imbalanced oxidative homeostasis,changed expression level of electron transport chain complexes,blocked tricarboxylic acid cycle,and insufficient ATP level.
