Effect of low intensity pulsed ultrasound on the migration and phagocytosis of lipopolysaccharide-induced RAW264.7 macrophages
10.12007/j.issn.0258-4646.2024.01.003
- VernacularTitle:低强度脉冲超声对脂多糖诱导炎性活化的RAW264.7巨噬细胞迁徙和吞噬的影响
- Author:
Yumeng GUO
1
;
Yue WANG
;
Xiaohan LIU
;
Lin WU
Author Information
1. 中国医科大学口腔医学院·附属口腔医院修复科,辽宁省口腔疾病重点实验室,沈阳 110002
- Keywords:
low intensity pulsed ultrasound;
lipopolysaccharide;
macrophage;
migration;
phagocytosis
- From:
Journal of China Medical University
2024;53(1):15-19,33
- CountryChina
- Language:Chinese
-
Abstract:
Objective Tto evaluate the effect of low intensity pulsed ultrasound(LIPUS)on the migration and phagocytosis ability of lipopolysaccharide(LPS)-induced RAW264.7 macrophages and on macrophage behavior under inflammation.Methods An in vitro activated RAW264.7 macrophage model was developed using LPS.Cell viability was assessed using a CCK-8 assay to explore the effect of LIPUS on activated and inactivated macrophages.Wound healing assays were employed to measure the effect of LIPUS on macrophage migration,and the Transwell assay was employed when LPS was used as a chemoattractant.Phagocytosis ability was examined using confocal microscopy and flow cytometry by observing the FITC fluorescence signal of internalized pHrodo Green E.coli BioParticles Conjugate.Results An activated RAW264.7 macrophage model was successfully developed using 100 ng/mL LPS.LIPUS inhibited the migration of inactivated macrophages into scratch areas as well as guided cell migration.However,cell viability and phagocytosis remained unchanged.Conclusion LIPUS may inhibit RAW264.7 migration but not affect the phagocytosis ability of the macrophages.
