Effects of bisphenol A and its three analogs on triglyceride synthesis in hepatocytes
10.12007/j.issn.0258-4646.2023.12.003
- VernacularTitle:双酚A及其3种替代物对肝细胞甘油三酯合成的影响
- Author:
Yang CHEN
1
;
Wenting GAO
;
Xiao LI
;
Xiaotong JI
;
Jianying BAI
Author Information
1. 山西医科大学公共卫生学院环境卫生学教研室,太原 030001
- Keywords:
bisphenol A;
bisphenol A analogues;
synthesis of triglyceride
- From:
Journal of China Medical University
2023;52(12):1068-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether BPA and its substitutes BPAF,BPB,and BPS can induce lipid accumulation in hepato-cytes.Methods HL-7702 cells were treated with BPA,BPAF,BPB,or BPS for 24 h.Lipid droplet accumulation in cells was observed via oil red O staining,and triglyceride content was determined through the GPO-PAP chemical-enzymatic method.In addition,qRT-PCR was used to detect the expression of triglyceride synthesis-related genes.Results Red lipid droplets of different sizes could be observed in the BPA,BPAF,BPB,and BPS groups.The intracellular triglyceride content increased significantly under 1 and 50 μmol/L BPA,10 and 50μmol/L BPAF,1,10,and 50μmol/L BPB,and 50μmol/L BPS.LIPIN2 mRNA expression level increased significantly under 10μmol/L BPAF,10μmol/L BPB,and 1 and 10μmol/L BPS.DGAT2 mRNA expression increased significantly under 50μmol/L BPA,10μmol/L BPAF,10 and 50μmol/L BPB,and 1μmol/L BPS.Conclusion BPAF,BPB,and BPS can increase intracellular triglyceride synthesis by upregulating LIPIN2 and DGAT2 mRNA expression,leading to intracellular fat accumulation and increased triglyceride con-tent in HL-7702 cells.
