Protective effect of dexmedetomidine on pathological cardiomyocyte hypertrophy
10.12007/j.issn.0258-4646.2023.12.001
- VernacularTitle:右美托咪定对病理性心肌细胞肥大的保护作用
- Author:
Xuefeng CAO
1
;
Liang ZHAO
;
Bo FANG
;
Xudong LIU
;
Fengmei DUAN
;
Yunfei JI
Author Information
1. 承德医学院附属医院麻醉科,河北 承德 067000
- Keywords:
dexmedetomidine;
cardiac hypertrophy;
angiotensin Ⅱ;
cell protection
- From:
Journal of China Medical University
2023;52(12):1057-1061
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this study was to investigate the protective effect of dexmedetomidine(DEX)on pathological car-diomyocyte hypertrophy.Methods An in vitro cell population was established in neonatal rats.The rats were divided into six groups:control group(C)without serum for 24 h,model group(A)with angiotensin Ⅱ(Ang Ⅱ)for 24 h,dexmedetomidine group(AD)with Ang Ⅱ+DEX(5μmol/L)for 24 h,C'group with serum-free culture for 48 h,A'group with Ang Ⅱfor 24 h,and AD'group with DEX+Ang Ⅱfor 24 h.The morphological changes of cells were observed by immunofluorescence.The protein expressions of atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP),and myosin heavy chain(β-MHC)were detected by western blot,and the cell activity was detected by CCK-8.Results Compared with group C,the size of cells in group A was larger,and that in group AD was even more significant.Simi-lar observations were found for hypertrophy related proteins.Compared with group C,the expression of ANP,BNP,and βMHC increased in group A,although the increase in AD group was more obvious.CCK-8 detection showed that compared with group C,the activity of group A decreased and that of group AD increased significantly.Compared with the C'group,the expression of hypertrophy-related pro-tein in the A'group was significantly increased,but the expression of ANP and BNP protein in the AD'group was significantly lower than that in the A'group.The differences were statistically significant(P<0.05).Conclusion Dexmedetomidine can alleviate the occur-rence of pathological hypertrophy through compensatory mechanisms similar to physiological myocardial hypertrophy,and may play a role in myocardial protection.
