Liraglutide protects nucleus pulposus cells by inhibiting the activation of NLRP3 inflammasome in high glucose environment
10.3969/j.issn.1006-6187.2024.01.010
- VernacularTitle:利拉鲁肽通过抑制高糖环境下NOD样受体家族核苷酸结合寡聚化结构域样受体3炎性体活化保护人髓核细胞的研究
- Author:
Mingyan YAO
1
;
Jing ZHANG
;
Shuqin GUO
Author Information
1. 071000 保定市第一中心医院内分泌科
- Keywords:
NLRP3;
Liraglutide;
Nucleus pulposus cells;
High glucose
- From:
Chinese Journal of Diabetes
2024;32(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effect of human nucleus pulposus cell by Liraglutide(Lir)under high-glucose environment via inhibition of NOD-like receptor family,pyrin domain containing 3(NLRP3)inflammasome activation and its mechanism.Methods Human nucleus pulposus cell lines were cultured and the third-generation nucleus pulposus cells were randomly divided into control group(Con group),high glucose group(HG group),and Liraglutide interference group(Lir group),and cultured for 48 h.ELISA was used to detect interleukin IL-1β;flow cytometry was used to testreactive oxygen species(ROS),and Western blot was used to evaluate the protein expressions of NLRP3,Pro-caspase-1,and Caspase-1.Results Compared with Con group,IL-1β,ROS,NLRP3,Pro-caspase-1,Caspase-1 protein expression and cell apoptosis were increased in HG and Lir groups(P<0.05).Compared with HG group,IL-1β,ROS,NLRP3,Pro-caspase-1,Caspase-1 protein expression and cell apoptosis were significantly decreased in Lir group(P<0.05).Conclusion Liraglutide protects human NPCs by inhibiting the activation of NLRP3 inflammasome,reducing IL-1β secretion,and inhibiting cell apoptosis.
