Changes of Th1 and B cells and related genes in diabetic foot ulcer healing
10.3969/j.issn.1006-6187.2024.01.007
- VernacularTitle:糖尿病足溃疡愈合中Th1和B细胞以及相关基因表达变化的研究
- Author:
Lei REN
1
;
Yifeng CHENG
;
Yang ZHAO
Author Information
1. 830011 乌鲁木齐,新疆医科大学第一附属医院烧伤科
- Keywords:
Diabetic foot ulcer;
Th1 cell;
B cell;
Gene expression
- From:
Chinese Journal of Diabetes
2024;32(1):29-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes in Th1 cell,B cell and related gene expression during the healing of diabetic foot ulcers(DFU).Methods Lesional skin tissues from DFU patients surgically treated in our hospital from January 2022 to January 2023 were selected.DFU associated gene expression data were collected from GSE80178 and GSE143735 datasets.The expressions of genes(DEGs)between ulcer and no-ulcer patients were identified.Bioinformatics methods were used to identify abnormal immune cells and key genes.Finally,the key results were detected by RT-qPCR and flow cytometry.Results A total of 548 common DEGs were identified in two datasets.In 14 co-expression modules,darkgrey and darkgreen were most related to ulcer,which were mainly associated with the regulation of enzyme activity,immune function and endocrine regulation.Flow cytometry showed that Th1 and B cells were highly infiltrated in ulcer tissue and decreased in healing tissue.MMP13,S100A9 and STAT4 were involved in immune signaling pathways.MMP13 and STAT4 were lowly expressed in healed ulcers,whereas S100A9 was highly expressed.Conclusion Reduced levels of Th1 and B cell infiltration may promote DFU healing.
