Establishment of a genetic monitoring method for laboratory quails
10.3969/j.issn.1671-7856.2024.02.012
- VernacularTitle:实验用鹌鹑遗传检测方法的初步建立
- Author:
Yang HE
1
,
2
;
Xiulin ZHANG
;
Qiuyu ZHANG
;
Xiaolu ZHANG
;
Bo FU
;
Wendong WANG
;
Xiaoyan DU
;
Zhenwen CHEN
;
Changlong LI
Author Information
1. 大连医科大学护理学院,辽宁大连 116044
2. 首都医科大学基础医学院,北京 100069
- Keywords:
laboratory quails;
genetic monitoring method;
microsatellite;
closed colony
- From:
Chinese Journal of Comparative Medicine
2024;34(2):101-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a genetic monitoring method for laboratory quails.Methods Quail microsatellite loci were searched in the literature,and microsatellite DNA loci suitable for quails were screened by an interspecific transfer method in closely related species,namely chickens and ducks.Quail liver DNA was extracted as a template,and the corresponding loci were screened by PCR amplification and agarose gel electrophoresis.On the basis of amplification of the selected microsatellite loci,the number of alleles,polymorphisms,and microsatellite loci combinations for quail genetic quality detection were selected and detection method were developed.Results We preliminary determined 23 microsatellite loci for genetic monitoring of closed-colony laboratory quails.Conclusions A genetic monitoring method for laboratory quails was preliminary developed.
