Osteopontin activates the PI3K/AKT pathway by upregulating LGALS3BP in promotion of hepatoma cell migration
10.3969/j.issn.1671-7856.2024.02.002
- VernacularTitle:骨桥蛋白通过LGALS3BP调控PI3K/AKT通路促进肝癌细胞迁移
- Author:
Linlin DENG
1
;
Riwen AN
;
Fangxin ZHAO
;
Ting LIN
;
Cuihua LIU
;
Mei HONG
;
Jianqiang WU
;
Xuan ZHANG
Author Information
1. 内蒙古医科大学基础医学院,呼和浩特 010059
- Keywords:
osteopontin;
LGALS3BP;
PI3K/AKT pathway;
liver cancer;
cell migration
- From:
Chinese Journal of Comparative Medicine
2024;34(2):9-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of osteopontin(OPN)in hepatoma cell migration through galectin-3 binding protein(LGALS3BP).Methods Human hepatoma cell lines SMMC-7721,SMMC-P(stably transfected with empty eukaryotic expression vectors),and SMMC-OPN(stably transfected with the OPN gene)were cultured.mRNA expression levels of OPN and LGALS3BP were measured by RT-qPCR.Western blot assays were used to analyze the relative protein expression of OPN and LGALS3BP and PI3K/AKT pathway.Wound healing assays were performed to explore the cell migration ability.After transfection with LGALS3BP-targeting small interfering RNA(si-LGALS3BP)or negative control small RNA(si-NC)into SMMC-OPN cells,cell migration and relative expression of PI3K/AKT pathway-related proteins were assessed.Results Compared with SMMC-7721 and SMMC-P,the migratory ability of SMMC-OPN cells was significantly reinforced,and expression of LGALS3BP was obviously upregulated at both mRNA and protein levels.Moreover,relative expression of p-PI3K/PI3K and p-AKT/AKT proteins was significantly increased.Wound healing assays showed that the si-LGALS3BP obviously suppressed the migratory ability of SMMC-OPN cells.Furthermore,relative expression of p-PI3K/PI3K and p-AKT/AKT proteins in SMMC-OPN cells was significantly decreased after transfection of si-LGALS3BP.Conclusions OPN activates the PI3K/AKT pathway by upregulating LGALS3BP expression to promote hepatoma cell migration.
