Exploring the therapeutic effect of licorice zinc on chloasma mice via the Nrf-2/HO-1 pathway
10.3969/j.issn.1671-7856.2024.01.009
- VernacularTitle:基于Nrf-2/HO-1探究甘草锌对黄褐斑小鼠的治疗作用
- Author:
Xiaoshuang DU
1
;
Ping LIU
;
Ying DENG
;
Hongqiu YANG
;
Yu DU
Author Information
1. 西南医科大学附属中医医院医学美容中心,四川泸州 646000
- Keywords:
licorice zinc;
Nrf-2/HO-1 pathway;
chloasma;
oxidative stress;
inflammation
- From:
Chinese Journal of Comparative Medicine
2024;34(1):80-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of licorice zinc on melasma.Methods Thirty-six BALB/c mice were equally divided into blank group,model group,licorzinc low-dose group,licorzine medium-dose group,licorzinc high-dose group and tranexamic acid group.Melasma was induced by 100 mJ/cm2 UVB irradiation combined with 15 mg/kg progesterone injection.Mice were treated with tranexamic acid(0.065 g/kg)and low(0.65 g/kg),medium(1.3 g/kg),or high(2.6 g/kg)doses of zinc licorice for 14 days.Skin was taken for HE and Masson-Fontana staining and measurement of SOD,MDA,GSP-Px,TNF-α,IL-1β,IL-6,plasma protein Nrf-2,nuclear protein Nrf-2 and HO-1 expression levels.Results Compared with model group,high-dose licorice zinc group showed decreased melanocyte formation,collagen cell necrosis,and inflammatory infiltration(P＜0.01);decreased MDA,IL-6,IL-1β,TNF-α and plasma protein Nrf-2 expression(P＜0.01);and increased GSP-Px,SOD and nuclear protein Nrf-2 and HO-1 expression(P＜0.01).Conclusions Zinc licorice activates the Nrf-2/HO-1 pathway to initiate high expression of HO-1,SOD and GSP-Px and fight oxidative stress,thereby reducing melanogenesis.
