Effects of emodin on autophagy in fibroblast-like synoviocytes in rheumatoid arthritis
10.3969/j.issn.1671-7856.2024.01.001
- VernacularTitle:大黄素对人类风湿关节炎成纤维样滑膜细胞自噬的影响
- Author:
Bonan TAO
1
;
Yonglan WANG
;
Lan HONG
;
Lin YUAN
Author Information
1. 湖北民族大学,风湿性疾病发生与干预湖北省重点实验室,湖北恩施 445000
- Keywords:
emodin;
MH7A cells;
cGAS/STING signaling pathway;
autophagy;
rheumatoid arthritis
- From:
Chinese Journal of Comparative Medicine
2024;34(1):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the cGAS/STING signaling pathway and investigate the potential effect of emodin(EMD)on autophagy of human rheumatoid arthritis fibroblast synovial cells(MH7A).Methods CCK-8 method was used to detect MH7A cell proliferation,and the experimental concentration of EMD was screened according to cell survival rate.Then,autophagy inhibitor 3-MA was added to further verify the effect of EMD on autophagy.Autophagy of MH7A cells was detected via the monodansylcadaverine staining method.Protein expression levels of cGAS,STING,p-STING,LC3-Ⅰ,LC3-Ⅱ,P62 and Beclin-1 were detected by Western blot.Results Monodansylcadaverine staining indicated that EMD enhanced the autophagy of MH7A cells.Western blot indicated that EMD decreased the expression of autophagy related proteins cGAS,STING,p-STING and P62,and increased that of LC3-Ⅱ and Beclin-1 in MH7A cells.After addition of the autophagy inhibitor 3-MA,the expression of P62 protein in MH7A cells increased,while that of LC3-Ⅱ and Beclin-1 decreased.Conclusions EMD may accelerate autophagy and inhibit MH7A cell proliferation by down-regulating cGAS/STING signaling pathway proteins.
