Establishment and evaluation of a rat model of coronary microvascular disease with qi deficiency and blood stasis syndrome
10.3969/j.issn.1005-4847.2023.12.002
- VernacularTitle:冠状动脉微血管疾病气虚血瘀证大鼠模型的建立及评价
- Author:
Jing KANG
1
,
2
;
Lili YANG
;
Ziyan WANG
;
Yue YOU
;
Yue SHI
;
Yanlei MA
;
Hongxu MENG
;
Lei LI
Author Information
1. 中国中医科学院西苑医院 中药药理北京市重点实验室,国家中医心血管疾病临床医学研究中心,北京 100091
2. 黑龙江中医药大学,哈尔滨 150040
- Keywords:
coronary microvascular disease;
qi deficiency and blood stasis syndrome;
combination of disease and syndrome;
sleep deprivation;
animal model
- From:
Acta Laboratorium Animalis Scientia Sinica
2023;31(12):1530-1538
- CountryChina
- Language:Chinese
-
Abstract:
Objective The incidence of coronary microvascular disease(CMVD)is increasing annually.According to traditional Chinese medicine(TCM),CMVD belongs to the category of"collaterals",and qi deficiency and blood stasis are the main syndrome type of CMVD.Notably however,no studies have reported on the use of animal models of CMVD with qi deficiency and blood stasis.The current study therefore aimed to establish and evaluate a rat model of CMVD with qi deficiency and blood stasis syndrome.Methods Forty-five male SD rats were divided randomly into sham group,CMVD group,and CMVD + QXXY group(n = 15 rats per group).Rats in the CMVD + QXXY group were randomly deprived of sleep for 14～16 h/day for 6 weeks,and the model of qi deficiency syndrome was established.Animals in the sham group and the CMVD group were fed normally for 6 weeks.After 6 weeks,rats in the CMVD and CMVD + QXXY groups were anesthetized,their chests were opened,and embolic microspheres(40～120 μm)were injected into the left ventricle.Rats in the sham group underwent thoracotomy without injection of embolic microspheres.On day 7 after operation,relevant detection indicators were measured in each group.Results Compared with the sham group,the CMVD group showed a significant decrease in left ventricular ejection fraction and left ventricular shortening rate,while the activities of creatine kinase MB isoenzyme(CK-MB)and lactate dehydrogenase(LDH)were significantly increased.Heart function,hemorheology,myocardial enzyme index,and the degree of myocardial cell damage differed significantly between the CMVD + QXXY group compared with the sham group.Conclusions A rat model of CMVD + qi deficiency + blood stasis syndrome can be successfully established by sleep deprivation combined with intraventricular injection of embolic microspheres.This model will be suitable for the study of the pathogenesis of CMVD and the mechanisms of TCM.
