Blood group serology and gene detection in patients with B(A) subgroup in Jiaozhou, Qingdao
10.13303/j.cjbt.issn.1004549x.2024.03.011
- VernacularTitle:青岛市胶州地区B(A)亚型患者的血型血清学及基因检测分析
- Author:
Hongwei QIN
1
,
2
;
Xiaoxia WANG
3
;
Ruilan YIN
3
;
Shuchao ZHANG
1
,
4
Author Information
1. Department of Immunology, College of Basic Medicine, Qingdao University, Qingdao 266071, China
2. Department of Blood Transfusion, Jiaozhou Central Hospital of Qingdao
3. Department of Blood Transfusion, Jiaozhou Central Hospital of Qingdao
4. Department of Blood Transfusion, the Affiliated Hospital of Qingdao University
- Publication Type:Journal Article
- Keywords:
ABO blood group;
B(A) subgroup;
blood group serology;
gene sequencing;
Qingdao
- From:
Chinese Journal of Blood Transfusion
2024;37(3):325-330
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate and analyze the serological and molecular biological characteristics of B(A) subgroup in a tertiary hospital in Jiaozhou, Qingdao. 【Methods】 From November 2019 to February 2023, the samples of 12 patients were suspected to be AB subgroup by microcolumn glass bead method and saline test tube method. The exons 6 and 7 of ABO gene were further amplified, sequenced and analyzed to determine the ABO allele type. 【Results】 A total of 9 cases of B(A) subgroup were detected in 26 065 patients in Jiaozhou, with a detection rate of 0.345 ‰ ( 9/26 065 ). Among the 9 cases of B(A) subgroup, 8 cases of serological reaction showed AweakB, and the gene detection was heterozygous for BA.04 gene and O gene.One case of serological reaction showed ABweak, and the gene detection was heterozygous for BA.04 gene and A gene. 【Conclusion】 Blood group serological combined with gene detection can accurately identify ABO blood group. B(A) subgroup alleles can exist in individuals with serological reaction of ABweak.
