Dentification and blood transfusion therapy of anti-C, e alloantibodies with anti-e, Jkb mimicking alloantibodies
10.13303/j.cjbt.issn.1004549x.2024.02.015
- VernacularTitle:同种抗-C、e伴类同种抗-e、Jkb自身抗体的鉴定及输血治疗
- Author:
Xin ZOU
1
;
Sifei MA
1
;
Hongmei YANG
1
;
Xiaojie FAN
2
Author Information
1. Changzhou Blood Center, Changzhou 213000, China
2. Department of Clinical Laboratory, Changzhou First People′s Hospital
- Publication Type:Journal Article
- Keywords:
antibody screening;
mimicking alloantibody;
absorption-elution;
genetic testing
- From:
Chinese Journal of Blood Transfusion
2024;37(2):214-218
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the differential diagnosis of 1 anti-C, e alloantibodies combined with anti-e, Jkb mimicking alloantibodies by absorption-elution test and titer integral method. 【Methods】 ABO, Rh and Kidd blood group antigens were identified by tube method. Two sets of panel cells were used for antibody screening and antibody specificity identification by saline method, polyamine method and microcolumn gel method.The antibody was further confirmed by multiple absorption-elution tests and titer integral method. RHCE and JK gene were sequenced by multiple PCR. 【Results】 Serological gene sequencing analysis showed that the ABO blood group of the patient was A type with Rh subtype ccDEE and was positive for direct antiglobulin test (DAT). Multiple absorption-elution tests and titer integral method demonstrated that the serum of the patient contained anti-C, e alloantibodies along with anti-e, Jkb mimicking autoantibodies and there were anti-e, Jkb mimicking autoantibodies on red blood cells(RBCs). According to gene sequencing analysis, there was G>C at exon 676 of the RHCE gene, and the remaining exons were not mutated, suggesting that the RHCE phenotype was ccEE. The 838 G/A heterozygote of exon 9 in JK gene, Jk blood group phenotype was Jk (a+ b+ ). Cross matched type A ccDEE and Jk(a+ b-) RBCs were transfused, and no adverse reactions occurred. 【Conclusion】 Serology combined with molecular biology to identify the phenotype of the patient′s RBCs, absorption-elution test and titer integral method to identify the antibody of the patient′s serum can detect the alloantibody type, thus providing strategies for targeted blood transfusion.
