Retest improvement for minipool nucleic acid testing positive samples
10.13303/j.cjbt.issn.1004549x.2024.02.011
- VernacularTitle:改进核酸筛查混检阳性池再检模式的探讨
- Author:
Yun HOU
1
;
Qiuxia FENG
1
;
Bei LI
1
;
Longmu ZHANG
1
;
Zhongsi YANG
1
;
Shuxian JIAO
1
Author Information
1. Qingdao Blood Center, Qingdao 266071, China
- Publication Type:Journal Article
- Keywords:
hepatitis B virus(HBV);
nucleic acid testing(NAT);
minipool testing;
residual risk
- From:
Chinese Journal of Blood Transfusion
2024;37(2):190-195
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the correlation between the distribution interval of minipool nucleic acid testing(NAT) positive CT value and the resolution rate, so as to improve the retest model and reduce residual risk of blood transfusion. 【Methods】 The resolution testing results by Cobas S201 system of our blood center from January 2017 to December 2021 were retrospective analyzed, and the retest model was developed based on the distribution interval of CT values. For minipool NAT HBV positive samples from March 2022 to March 2023, synchronous detection was conducted by Cobas S201 and Panther detection system, and the detection results were statistically analyzed. 【Results】 From 2017 to 2021, 474 were minipool NAT positive, among which 324 were HBV positive, accounting for 68.35%. From 2017 to 2020, the proportion of HBV positive per year was significantly higher than that of HCV and HIV(P<0.05). In resolution testing, 167 were HBV repeatable positive and 157 were HBV non-repeatable positive, accounting for 51.54% and 48.46% of HBV minipool NAT positive. HBV repeatable positive samples were with three intervals: CT value≤36, 3640, with the resolution rate at 95.8%, 56.5% and 14.8% respectively(P<0.05). The proportion of 3640, 36
