Hemolytic diseases of the fetus and newborn caused by anti-Dib: a case report and related research
10.13303/j.cjbt.issn.1004549x.2024.02.006
- VernacularTitle:罕见抗-Dib致严重胎儿新生儿溶血病的实验室检测与相关研究
- Author:
Zhijian LIAO
1
;
Shuangshuang JIA
1
;
Jizhi WEN
1
;
Chunyan MO
1
;
Yuan SHAO
1
;
Runqing ZHANG
1
;
Guangping LUO
1
;
Yanli JI
1
Author Information
1. Institute of Clinical Blood Transfusion, Guangzhou Blood Center, Guangzhou 510095, China
- Publication Type:Journal Article
- Keywords:
anti-Dib;
Di(b-) rare blood group;
hemolytic disease of the fetus and newborn(HDFN)
- From:
Chinese Journal of Blood Transfusion
2024;37(2):158-164
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To identify the specificity of alloantibody against high-frequency antigens in one case suffering with severe hemolytic diseases of the fetus and newborn (HDFN) and to screen for matching blood for transfusion. 【Methods】 The HDFN test and the antibody serological identification tests in the mother were performed. Several common high frequency antigens of maternal red blood cells (RBCs) were determined. IgG subtype coated on the RBCs of the newborn was determined. The phagocytic efficiency of the antibody was tested using the monocyte phagocytosis of sensitized erythrocyte by flow cytometry in vitro. Sanger sequencing of DI gene was performed in the mother, father and mother’s brother. The diluted maternal plasma was used for large scale screening of matching blood using IAT in Coomb’s gel card. 【Results】 Di(b-) phenotype was identified in the mother of the newborn and anti-Dib (titer: 512) related HDN was detected in the newborn. IgG1 and IgG2 subtypes of anti-Dib were detected and the rate of monocyte phagocytosis was 88.83%(74.7/84.09). The compatible blood was not detected in the maternal relatives. Subsequently, the newborn received the matching RBCs of two Di(b-) donors identified from 5 520 blood donors and discharged from the hospital. We screened out 17 Di(b-) donors out of 51 334 blood donors, indicating that the distribution frequency of Di(b-) among blood donors in Guangzhou was about 0.033% (17/51 334). 【Conclusion】 By serology and molecular biology methods, the newborn was identified with HDFN caused by anti-Dib, and an effective large-scale screening method for Di (b -) rare blood types was established to find matching blood, which supported the establishment of rare Di(b-) blood database.
