Effect of long-term frozen plasma on the detection of HBsAg by ELISA

VernacularTitle:长期冷冻对ELISA法检测血浆标本中HBsAg结果的影响
Author: Simin LIAN ¹ ; Xiaohua CHEN ¹ ; Shubo LIN ¹ ; Ziyue GUO ¹ ; Zebin XIAO ¹
Author Information

1. Shantou Blood Station, Shantou 515064, China
Publication Type:Journal Article
Keywords: HBsAg; S/CO value; frozen plasma sample; blood donor
From: Chinese Journal of Blood Transfusion 2024;37(1):91-94
CountryChina
Language:Chinese
Abstract: 【Objective】 To evaluate the HBsAg detection results of HBsAg+ samples after 8 years refrigeration by ELISA and evaluate the effectiveness of the current storage policy of retained samples. 【Methods】 A total of 100 HBsAg+ plasma samples by ELISA from May 2014 to March 2015 and refrigerated at -20℃ were collected and retested for HBsAg using the same method after thawing in 2023. 【Results】 The HBsAg retest results of 100 plasma samples were all positive, with the concordance rate of 100%, though there was a significant decrease in the S/CO value after refrigeration(27.52 vs 19.03, P<0.05). 【Conclusion】 Long-term refrigeration can lead to a decrease in the S/CO value of HBsAg ELISA detection,but it does not affect the positive results.