Distribution of platelet antibodies and their specificity in Zhongshan area
10.13303/j.cjbt.issn.1004-549x.2024.01.011
- VernacularTitle:中山地区血小板抗体及特异性分布调查
- Author:
Huiyan LIN
1
;
Yonglun WU
1
;
Ainong SUN
1
;
Yuru FANG
1
;
Qianying CHEN
1
;
Qiao LI
1
;
Yujue WANG
2
;
Hongmei WANG
2
;
Zhizhao YANG
3
;
Xiaoyi JIAN
4
;
Xianguo XU
5
;
Shengbao DUAN
2
Author Information
1. Zhongshan Blood Center, Zhongshan 528400, China
2. Suzhou Institute of Biomedical Engineering and Technology
3. Zhongshan People's Hospital
4. Zhongshan Boai Hospital
5. Zhejiang Provincial Blood Center
- Publication Type:Journal Article
- Keywords:
platelet antibodies;
platelet transfusion refractoriness;
anti-CD36;
voluntary blood donors;
platelet cross matching(PXM)
- From:
Chinese Journal of Blood Transfusion
2024;37(1):63-67
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the frequency of platelet antibodies in voluntary blood donors and patients in Zhongshan, Guangdong Province, and to study the specificity and cross-matching of platelet antibodies. 【Methods】 Platelet antibodies of blood donors and patients were screened by solid-phase immunoadsorption (SPIA), rechecked by flow cytometry (FCM), and antibody specificity was identified by PakPlus enzyme immunoassay, and platelet cross-matching was simulated by SPIA. 【Results】 A total of 1 049 blood donor samples and 598 patient samples were tested, with 6 (0.57%) and 49 (8.19%) samples positive for SPIA,respectively(P<0.05); In SPIA positive samples, the positive concordance rate of FCM in blood donors and patients was 100% vs 95%, and that of enzyme immunoassay was 100% vs 88%. Among the initial screening positive samples of blood donors, 5 were anti-HLA Ⅰ antibodies, accounting for 83%, and 1 was anti CD36 antibody, accounting for 17%, with an incidence rate of 0.10%. Among the 14 samples of enzyme immunoassay positive patients, 2 were anti-GP Ⅱb/Ⅲa, 1 was anti-GP Ⅱa/Ⅱa, 8 were anti HLA Ⅰ, and 3 were mixed antibodies (HLA Ⅰ, GP Ⅱb/Ⅲa, GP Ⅰa/Ⅱa). According to the types of antibodies, HLA Ⅰ antibodies were the most common, accounting for 65% (11/17), followed by HPA related anti GP, accounting for 35% (6/17). The majority of patients had a platelet antibody positive typing rate below 30%, accounting for 71.4% (10/14). 【Conclusions】 The positive rate of platelet antibody of patients in Zhongshan area is significantly higher than that of voluntary blood donors, and most of them are anti-HLA Ⅰ and anti-GP, and the incidence of anti-CD36 is extremely low. Therefore, it is necessary to establish a known platelet antigen donor bank, and at the same time, carry out platelet antibody testing and matching of patients, which is helpful to solve the issue of platelet transfusion refractoriness.
