Characteristics of HBcrAg in HBsAg-/HBV DNA+ blood donors in Wuxi area
10.13303/j.cjbt.issn.1004-549x.2024.01.006
- VernacularTitle:无锡地区HBsAg-/HBV DNA+献血人群HBcrAg检出特点分析
- Author:
Yan WANG
1
;
Jianhuai JIN
1
;
Youshan XU
1
;
Qingqin HAO
1
;
Wei XIA
1
Author Information
1. Wuxi Central Blood Station,Wuxi 214000, China
- Publication Type:Journal Article
- Keywords:
hepatitis B core-related antigen (HBcrAg);
HBsAg-/HBV DNA+;
blood donors;
covalently closed circular DNA(cccDNA)
- From:
Chinese Journal of Blood Transfusion
2024;37(1):32-36
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the detection characteristics of a novel serum marker, hepatitis B core-associated antigen (HBcrAg), in the HBsAg-/HBV DNA+ blood donors in Wuxi. 【Methods】 A total of 37 previous HBsAg-/HBV DNA+ blood donors were followed up by telephone and their serum was obtained, and the serum of 22 HBsAg-/HBV DNA+ blood donors was detected by electrochemiluminescence and real-time PCR nucleic acid screening as the OBI group for HBcrAg enzyme-linked immunosorbent assay(ELISA). The serum of 20 healthy blood donors who underwent dual ELISA and one nucleic acid testing(NAT) was selected as the healthy control group, and the serum of 20 patients with chronic hepatitis B who were clinically diagnosed by Wuxi Fifth People's Hospital was selected as the experimental CHB group, and HBcrAg ELISA was detected respectively. The correlation analysis between HBcrAg and HBeAb, HBcAb, ALT and HBV DNA in the OBI group was performed. 【Results】 Thirty-seven blood samples were detected by chemiluminescence for HBsAg and NAT, and 22 HBsAg-/HBV DNA+ samples were detected in the OBI group, with a detection rate of 59.46%. The serum HBcrAg expression content (ng/mL) between the OBI group, the healthy control group and the CHB group were (0.92±0.13), (0.47±0.09) and (1.14±0.23), respectively, and the differences were statistically significant (P<0.05), the expression of HBcrAg in the OBI group and CHB group was higher than that in the healthy control group (P<0.05). There was no correlation between HBcrAg and HBeAb, HBcAb, ALT and HBV DNA indexes in the OBI group (P>0.05). 【Conclusion】 The expression of HBcrAg in the OBI group and CHB group was higher than that in the healthy control group, and the serum HBcrAg was not correlated with HBeAb, HBcAb, ALT and HBV DNA to a certain extent. HBcrAg has a good application prospect in screening HBsAg-/HBV DNA+ blood donors.
