Virulence determinants and genetic diversity of foodborne Yersinia enterocolitica isolated from Wenzhou
10.3969/j.issn.1002-2694.2024.00.011
- VernacularTitle:温州市食源性小肠结肠炎耶尔森氏菌毒力基因分布及遗传多样性研究
- Author:
Ai-Rong XIE
1
;
Yi LI
;
Hui-Huang LOU
;
Zhong-Bi XIE
;
Le-Yi ZHANG
;
Yu-Qin HU
;
Yue-Jin WU
Author Information
1. 温州市疾病预防控制中心,温州 325000
- Keywords:
Yersinia enterocolitica;
serotype;
virulence gene;
drug resistance;
whole genome sequencing
- From:
Chinese Journal of Zoonoses
2024;40(1):40-45
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the virulence determinants and genetic diversity of foodborne Yersinia enterocolitica from Wenzhou.A total of 71 strains of Yersinia enterocolitica were isolated from food and foodborne diarrhea ca-ses in Wenzhou,and their biotypes,serotypes,and drug resistance were analyzed.On the basis of whole genome sequencing,we assessed virulence gene profiles,and performed multilocus sequence typing(MLST)and core gene multilocus sequence typ-ing(cgMLST).A total of 94.4％(67/71)of isolates belonged to biotype 1A,and the highest proportion had serotype lA/O∶5(29.6％,21/71).The sensitivity rates of the isolates to 14 antibiotics exceeded 95.8％.A total of 16 categories and 126 viru-lence genes were identified,with two strains carrying the pYV plasmid and chromosome-related virulence genes.ST3(31.6％,12/38)was the most widespread MLST type,and cgMLST analysis revealed no dense clusters of genotypes except for strains sharing the same ST.In conclusion,pathogenic strains were identified from foodborne Yersinia enterocolitica in Wenzhou and were found to exhibit high genetic polymorphism.Enhanced regulatory supervision is essential to prevent the outbreak of food-borne diseases caused by Yersinia enterocolitica.
