Bioinformatics analysis of Brucella periplasmic protein EipB and its immune response characteristics of dominant T-B combined epitope
10.3969/j.issn.1002-2694.2023.00.140
- VernacularTitle:布氏杆菌周质蛋白EipB优势T-B联合抗原表位预测及其免疫应答特点
- Author:
Wei GOU
1
;
Shan CHEN
;
Wei HE
Author Information
1. 宁夏医科大学总医院心脑血管病医院血管外科,银川 750002
- Keywords:
brucellosis;
Brucella periplasmic protein;
epitope of antigen
- From:
Chinese Journal of Zoonoses
2023;39(12):1181-1187
- CountryChina
- Language:Chinese
-
Abstract:
Using bioinformatics methods to predict the physical and chemical properties,spatial structure,and T and B cell epitopes of Brucella Periplasmic Protein(EipB),and to explore the immune effects of T and B dominant epitope peptides,for Brucella Periplasmic Protein(EipB)Lay the foundation for the screening of molecular peptide vaccine against bacillosis.Online websites and bioinformatics-related databases were used to analyse the physicochemical properties,transmembrane structural domains,signal peptide sequences,secondary and tertiary structures,hydrophilicity/hydrophobicity and T and B antigenic epitopes of the EipB protein.And select the B and T epitopes with the highest antigenicity(located at 121-137 and 95-110 respectively)to connect with hemocyanin,and after immunizing the mice,detect the production of IgG,IgM,IgA and IgE an-tibodies by ELISA;Flow cytometry was used to detect the secretion of cytokines in T cells;The brucellosis EipB protein is composed of 279 amino acids,is weakly basic,has a molecular mass of 31 kDa and is relatively stable;contains a signal peptide sequence and a transmembrane structural domain;has a secondary structure with a-helix,β-turn,extended linkage and irregular coi-ling;has a distinct hydrophilic region,and the protein contains 21 dominant B-cell epitope peptides in amino acid regions,the amino acid regions in which these dominant B-cell epitopes are located correspond to regions of higher hydrophilicity.Contains 56 CD4+T cell epitopes and 39 CD8+T cell epitopes.After immunizing mice with T-B dominant cell epitope combination,antibodies such as IgG,IgM,IgA and IgE in serum increased significantly;T cells produced IFN-γ and other cytokines significantly increased.1)Bioinformatics method was used to predict that EipB pro-tein contains dominant B and T cell epitopes.2)Combined immunization of mice with dominant B-T dominant epitope peptides can induce a higher level of immune response in mice,providing a reference for the screening of brucellosis molecular peptide vaccines.
