Three molecular typing methods for Vibrio parahaemolyticus
10.3969/j.issn.1002-2694.2023.00.131
- VernacularTitle:副溶血性弧菌的3种分子分型方法研究
- Author:
Xue LI
1
;
Ting-Ting SUN
;
Tong-Zhu WEI
;
Wei-Jie WANG
;
Wen-Li DIAO
Author Information
1. 辽宁省疾病预防控制中心,沈阳 110005
- Keywords:
Vibrio parahaemolyticus;
molecular typing;
serum typing;
kinship;
traceability
- From:
Chinese Journal of Zoonoses
2023;39(11):1107-1111
- CountryChina
- Language:Chinese
-
Abstract:
This study explored three molecular typing methods for Vibrio parahaemolyticus(VP)in Liaoning Province in 2020,to assess the correlation among the three methods and the genetic relationships among between strains;analyze the epi-demic trends and distribution patterns of VPin Liaoning Province;and provide reliable technical support for the prevention and control of foodborne diseases.Serum typing,PFGE,REP-PCR,and ERIC-PCR molecular typing and cluster analysis were performed on 44 VP isolates from Liaoning Province in 2020.A total of 44 isolated strains were divided into 15 serotypes,and 8 isolated strains could not be classified.The serotypes were primarily O3 group,O1 group,and O2 group.Clinical isolates had high molecular similarity,whereas food isolates had low molecular similarity.The resolution(DI)of PFGE was 0.986,that of REP-PCR was 0.947,and that of ERIC-PCR was 0.935.The molecular similarity between serotype O3 and O1 group strains was high.The epidemic serotypes of isolated VP strains in Liaoning Province in 2020 were consistent with those from the past 5 years.The resolution of the PFGE typing method was better than that of REP-PCR and ERIC-PCR;moreover,REP-PCR had better resolution than ERIC-PCR.These three typing methods showed good intercorrelation.The O3 group strains are likely to originate from the O1 group strains.When a foodborne disease outbreak is caused by VP,laboratories with conditions can apply these three methods to trace the source of the pathogenic bacteriaquickly and effectively.
