Effects of PD-1 blockade on macrophage phagocytosis after Echinococcus multilocularis protoscoleces infection

VernacularTitle:多房棘球蚴原头节感染后阻断PD-1对巨噬细胞吞噬功能的影响
Author: Tao ZHANG ^{1
,

2
,

3} ; Yao-Gang ZHANG ; Zi-Han YANG ; Yin-Hong SHEN ; Yan-Yan MA
Author Information

1. 青海大学附属医院,西宁 810000
2. 青海大学高原医学研究中心,西宁 810000
3. 青海省高原医学实验室,西宁 810000
Keywords: Echinococcus multilocularis protoscoleces; Programmed death(PD-1); macrophage; phagocytosis
From: Chinese Journal of Zoonoses 2023;39(11):1095-1099
CountryChina
Language:Chinese
Abstract: This study analyzed the changes in macrophage phagocytosis after Echinococcus multilocularis protoscoleces(PSC)infection.The distribution of macrophages in hepatic alveolar echinococcosis patients was analyzed by immunohisto-chemistry.PD-1 blockade,PSCs and macrophages were co-cultured to analyze macrophage phagocytosis and the expression lev-els of CD47 and PD-1.Macrophages labeled by CD68 aggregated in close live tissue from the lession of alveolar echinococcosis patients.PSC infection resulted in lower intensity of Escherichia coli and higher expression of CD47 and PD-1 than observed in the control group(P＜0.001).The intensity of Escherichia coli increasedin the PD-1 blockade group after PSC infection(q=40.63,P＜0.05).PD-1 blockade restored macrophage phagocytosis after PSC infection.