Observation on formation of neutrophil extracellular traps in peripheral blood of patients with psoriasis
10.3969/j.issn.1000-484X.2024.03.026
- VernacularTitle:对银屑病患者外周血中性粒细胞胞外诱捕网形成的观察
- Author:
Xiaoyan QU
1
;
Pan CHEN
;
Wenqiong NI
;
Hui XIAO
Author Information
1. 郑州大学附属郑州中心医院皮肤科,郑州 450007
- Keywords:
Psoriasis;
Neutrophil extracellular traps;
Disease severity;
Comorbidity
- From:
Chinese Journal of Immunology
2024;40(3):599-603,609
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical application value of neutrophil extracellular traps(NETs)in the evaluation of psoriasis patients.Methods:Peripheral blood(2 ml)of 63 patients with psoriasis and 27 healthy controls were collected,neutrophils were isolated by density gradient method,and the formation of NETs was observed by immunofluorescence staining.Calculated the pro-portion and fluorescence intensity of NETs,and analyzed their correlation with clinical classification,severity and serological indica-tors.Skin lesions of 5 patients with psoriasis were collected,and the NETs were observed by immunofluorescence method.Results:Proportion and fluorescence intensity of spontaneous NETs in patients with psoriasis were significantly higher than those in healthy con-trols,and were positively correlated with the PASI scores.Production of NETs in psoriasis patients with metabolic abnormalities or TNF-α abnormalities was higher than that in psoriasis patients without metabolic abnormalities or TNF-α normalcy.Expressions of NETs in psoriatic lesions were increased.Conclusion:Levels of NETs are correlated with disease severity of psoriasis and patients with metabolic abnormalities,and may be used as a clinical indicator to reflect the inflammatory state of psoriasis and metabolic comor-bidity of psoriasis for disease evaluation.
