Differential gene expression and immune cell infiltration analysis of gout gene expression profile
10.3969/j.issn.1000-484X.2024.03.025
- VernacularTitle:痛风基因表达谱的差异基因表达及免疫细胞浸润分析
- Author:
Feng CHEN
1
;
Huanan LI
;
Xiaoyun ZHANG
;
Huilian HUANG
;
Yueping CHEN
;
Guowu REN
Author Information
1. 广西中医药大学附属瑞康医院骨科,南宁 530011
- Keywords:
Gout;
Monosodium urate;
Bioinformatics;
Immune cells;
Cellular infiltration
- From:
Chinese Journal of Immunology
2024;40(3):592-598
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the differential gene expression and immune cell infiltration of gout patients,to find the key genes and immune cells of gout pathogenesis,and to explore the relationship between immune cells and gout.Methods:The gout chip GSE160170 was downloaded from the GEO database,and the differential gene expression analysis was carried out with the help of R language.Then,the STRING database was used to analyze the differential gene,and the Cytoscape software was used to screen the key genes,and then carry out enrichment analysis.At the same time,the infiltration of immune cells were analyzed.Results:The study found that IL-6,IL-1β,TNF,CCL3,CXCL8 and CXCL1 were key genes in the pathogenesis of gout,which were mainly exerted by IL-17,Toll-like receptor,NOD-like receptor,NF-κB and other signaling pathways.Processes such as cellular responses to lipo-polysaccharides,bacteria-derived molecules,and biological stimuli lead to disease;immune infiltration results indicate that memory B cells,activated NK cells,activated dendritic cells,activated mast cells and eosinophils were involved in the disease.It was signifi-cantly expressed in gout patients;the correlation analysis between immune cells showed that the expression of follicular helper T cells were positively correlated with the expression of activated mast cells,and the expression of unactivated NK cells and monocyte were negatively correlated.Conclusion:Key genes and differentially expressed immune cells are closely related to the pathogenesis of gout,providing new ideas for the study of the molecular mechanism of gout.
