Immune response and antitumor effects of HCA587/MAGE-C2 protein formu-lated with CFA and CpG
10.3969/j.issn.1000-484X.2024.03.022
- VernacularTitle:HCA587/MAGE-C2蛋白联合CFA和CpG佐剂的免疫应答及抗肿瘤效应
- Author:
Wei JIAO
1
;
Yuanfang TAN
;
Huiyuan CHEN
;
Qiuying FAN
;
Yanhui YIN
;
Juanjuan CHEN
Author Information
1. 南昌大学第二附属医院检验科,江西省检验医学重点实验室,南昌 330006
- Keywords:
HCA587 protein;
Adjuvant;
IFN-γ;
CD4+T cell;
Antitumor effect
- From:
Chinese Journal of Immunology
2024;40(3):572-576
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate ability of HCA587/MAGE-C2 protein combined with different adjuvants inducing antigen-specific immune response and antitumor effects in mice model.Methods:C57BL/6J mice were immunized with HCA587 protein com-bined with Freund's complete adjuvant(CFA)/Freund's incomplete adjuvant(IFA)and different doses of CpG ODN 1826(CpG),cellular and humoral immunity levels induced by different schemes were compared.ELISpot was used to evaluate frequency of IFN-γ-producing splenocytes.HCA587-specific antibodies were detected by ELISA.Intracellular cytokine staining(ICCS)analysis was mea-sured by flow cytometry.A tumor-bearing animal model was created by subcutaneously injection of B16-HCA587 tumor cells into right flank of C57BL/6J mice,which was treated with strategy with the strongest cellular and humoral immune response in immune compari-son protocol.Vernier calipers were used to measure tumor volume,and Log-rank test was used to analyze survival curve.Results:HCA587 protein combined with CFA and 50 μg CpG elicited strongest specific IFN-γ-secreting splenocytes and anti-HCA587 anti-bodies,which induced highest IFN-γ+CD4+T cells(P<0.05).In tumor treatment model,HCA587 protein combined with CFA and 50 μg CpG significantly inhibited tumor growth(P=0.026),while Log-rank test showed no significant effect on survival(P>0.05).Conclusion:HCA587 protein vaccine formulated with CFA and 50 μg CpG causes a significant cellular and humoral immune response and partial antitumor effect in mice model,providing new experimental data for preclinical research of tumor antigen protein vaccine.
