IL-10 inhibits expression of inflammatory factors in Chlamydia-infected cells by up-regulating socs3

VernacularTitle:IL-10通过上调socs3抑制衣原体感染细胞炎症因子表达
Author: Lingyan LUO ¹ ; Kun DU
Author Information

1. 长江大学附属第一医院输血科,荆州 434000
Keywords: Chlamydia trachomatis; Inflammatory factors; IL-10; SOCS
From: Chinese Journal of Immunology 2024;40(3):530-533
CountryChina
Language:Chinese
Abstract: Objective:To investigate the role and mechanisms of SOCS3 protein in IL-10 inhibiting the expression of inflam-matory factors in Chlamydia-infected cells.Methods:The activation of STAT3 protein were examined in Chlamydia-infected cells by Western blot,and the activation of p38 and ERK1/2 were also examined in infected cells treated with socs3 siRNA.The expression of socs3 gene was examined in Chlamydia-infected cells treated with IL-10 or Stattic by RT-PCR.IL-6 and IL-12 were measured in infect-ed cells treated with socs3 siRNA using ELISA kits.Results:Socs3 expression was up-regulated by IL-10 through activation of STAT3 protein.IL-6 and IL-12 induced by Chlamydia were down-regulated by IL-10 through induction of socs3.The activation of p38 and ERK1/2 signalling pathways were inhibited by SOCS3.Conclusion:IL-10 inhibits the production of pro-inflammatory cytokines through induction of SOCS3 and inhibition p38 and ERK1/2 signalling pathways.