Study on lipidomics of CD4+T cells in mice with diabetic kidney disease
10.3969/j.issn.1000-484X.2024.03.005
- VernacularTitle:糖尿病肾病小鼠CD4+T细胞的脂质组学研究
- Author:
Xiaoyu ZHANG
1
;
Haibo TAN
;
Minyi HUANG
;
Weijian BEI
;
Yiqi YANG
Author Information
1. 广东药科大学中医药研究院,广州 510006
- Keywords:
CD4+T cells;
Diabetic kidney disease;
Lipidomics;
Magnetic bead sorting;
Flow cytometry
- From:
Chinese Journal of Immunology
2024;40(3):471-477
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the lipidomics differences of CD4+T immune cells in diabetic kidney disease(DKD)mice,and screen out the differential metabolites with biological significance.Methods:CD4(L3T4)MicroBeads immunomagnetic beads were used to isolate CD4+T immune cells from spleen of BKS.Cg-Dock7m+/+Leprdb/J mice with spontaneous DKD;the purity of CD4+T cells were identified by flow cytometry.The non-targeted lipidomics of CD4+T cells were detected by LC-MS/MS,and the differ-ential metabolites were analyzed.Results:A total of 463 metabolites were detected by LC-MS.PCA and OPLS-DA analysis showed that the metabolic components were significantly separated;twenty-four differential metabolites were screened out.KEGG and enrich-ment analysis showed that the differential metabolites involved in the disorder of glycerol phospholipid metabolism.Conclusion:Phos-pholipid metabolism of CD4+T cells is closely related to the occurrence of DKD.Phospholipid metabolism targeting DKD CD4+T cells in DKD may be a new direction of DKD treatment.
