Interleukin-12 participate in liver diseases of Sj?gren's syndrome through promoting oxidative stress
10.3969/j.issn.1000-484X.2024.03.003
- VernacularTitle:白细胞介素-12促进氧化应激参与干燥综合征肝脏病变
- Author:
Jie ZHA
1
,
2
;
Tingting JIANG
;
Junqiao GUO
;
Zhen XIAO
;
Genhong YAO
Author Information
1. 南京鼓楼医院风湿免疫科,南京 210008
2. 安庆医药高等专科学校基础医学院,安庆 246052
- Keywords:
Sj?gren's syndrome;
Liver disease;
Interleukin-12;
Oxidative stress
- From:
Chinese Journal of Immunology
2024;40(3):461-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of interleukin-12(IL-12)on liver lesions and oxidative stress pathway in Sjo-gren's syndrome mice,and to clarify the possible mechanism of liver lesions in Sjogren's syndrome.Methods:Saliva flow rate,liver function,liver pathology and IL-12 level were measured in NOD,IL-12 knockout(IL-12KO)NOD and C57BL/6(B6)mice.Different concentrations of IL-12 and JAK2,TYK2 inhibitors were applied to mouse hepatoma cells.The oxidative stress index of mouse serum and cell culture supernatants of each group were determined.Results:Compared with the other two groups of mice,the NOD mice had significantly higher GOT and GPT(P<0.05),and decreased serum GSH-PX,SOD and CAT(P<0.05).CAT,GSH,GSH-PX and SOD were decreased,while MDA was increased in mice treated with different concentrations of IL-12.JAK2/TYK2 inhibitors reversed regulatory effects of IL-12 on SOD,GSH and MDA in hepatoma cells(P<0.05).Conclusion:IL-12 may aggravated liver damage of Sj?gren's syndrome through promoting oxidative stress of hepatocytes.
