Preparation and applications of monoclonal antibody against TREM2
10.3969/j.issn.1000-484X.2024.01.026
- VernacularTitle:TREM2单克隆抗体的制备及应用检测
- Author:
Bin MA
1
;
Lin MENG
;
Zhuona RONG
;
Chuanke ZHAO
;
Chengchao SHOU
Author Information
1. 北京大学临床肿瘤学院,北京大学肿瘤医院暨北京市肿瘤防治研究所,生物化学与分子生物学研究室,恶性肿瘤发病机制及转化研究教育部重点实验室,北京 100142
- Keywords:
TREM2;
Monoclonal antibody;
Preparation;
Identification
- From:
Chinese Journal of Immunology
2024;40(1):168-173,177
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To preparation TREM2-specific monoclonal antibodies and to evaluation their properties and applica-tions lay a foundation for the development of druggable antibodies against TREM2 targets.Methods:The GST fusion protein containing the extracellular segment of TREM2 was expressed and purified,which was used as antigen to immunize mice.The mouse with high antiserum titer was used to generate specific monoclonal antibodies against TREM2 through cell fusion and monoclonal screening.The specificity and affinity of the monoclonal antibodies and their applications in different immunological experiments were detected.Results:A total of 29 strains of anti-TREM2 monoclonal antibodies were obtained,of which 24 strains could specifically bind to TREM2.The EC50 of antibodies were calculated above nanomolar.These monoclonal antibodies could be used for specific detection of TREM2 in Western blot,immunoprecipitation,flow cytometry and immunofluorescence experiments.Conclusion:Monoclonal anti-bodies against TREM2 with high affinity and specificity are successfully generated,which lay the foundation for the research and the development of antibody drugs targeting TREM2.
