Role and mechanism of BRCA2 gene in immune killing of lung cancer cells
10.3969/j.issn.1000-484X.2024.01.020
- VernacularTitle:BRCA2基因在免疫杀伤肺癌细胞中的作用及机制研究
- Author:
Qungui ZHANG
1
;
Jianping LAI
;
Xiaojing LI
;
Li HUANG
Author Information
1. 赣州市肿瘤医院,赣州 341000
- Keywords:
RNAi technology;
BRCA2 gene;
Peripheral blood mononuclear cells;
Lung cancer
- From:
Chinese Journal of Immunology
2024;40(1):134-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect and molecular mechanism of BRCA2 gene on the killing of lung cancer by immune cells.Methods:siRNA was designed to reduce BRCA2 gene in lung cancer cells;BRCA2 expression was detected by qPCR and Western blot;cell growth was detected by MTT and CCK-8 methods;peripheral blood mononuclear cells were co-cultured with lung cancer cells,and GFP fluorescence was detected by enzyme labeling method.Killing efficiency of lung cancer cells was evaluated.Results:BRCA2 gene was expressed in moderate abundance in lung cancer cells A549 and H1299,and there was no significant differ-ence compared with lung epithelial cells BEAS-2B(P>0.05).When the BRCA2 gene in A549 and H1299 cells was successfully knocked down,the cell proliferation rate was significantly increased compared with control group;the killing efficiency of peripheral blood mononuclear cells to lung cancer cells A549 and H1299 were significantly higher than that of the control group;the expression of ATM,RAD51 and RAD50 were significantly reduced,while the expression of P53 protein was 7.2 times of the control group.Con-clusion:After knockdown of BRCA2 gene,peripheral blood monocytes are more effective in killing lung cancer cells.Intervention of BRCA2 and monocytes can synergistically inhibit lung cancer and regulate the expression of ATM signaling pathway molecules.
