Expression and clinical significance of long non-coding RNA ZIM2-AS1 in hepatocellular carcinoma
10.3969/j.issn.1000-484X.2024.01.017
- VernacularTitle:长链非编码RNA ZIM2-AS1在肝细胞癌中的表达及其临床意义
- Author:
Jin SUN
1
;
Yingnan LI
;
Mengjiao SHI
;
Hongwei TIAN
;
Yanhua MU
;
Jun LI
;
Zongfang LI
Author Information
1. 西安交通大学第二附属医院生物诊断治疗国家地方联合工程研究中心,西安 710004
- Keywords:
Long non-coding RNA;
ZIM2-AS1;
Hepatocellular carcinoma;
Prognosis;
Diagnosis;
Immune cell infiltration
- From:
Chinese Journal of Immunology
2024;40(1):116-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of long non-coding RNA(lncRNA)ZIM2-AS1 in hepatocellular carcinoma(HCC)and its clinical significance as well as diagnostic value using the data obtained from the Cancer Genome Atlas(TCGA).Meth-ods:The transcriptome sequencing(RNA-seq)data and clinical information of 374 HCC tissues and 50 paired paracancerous tissues were gathered from the TCGA database,then the expression trends of ZIM2-AS1 in HCC and its correlation with clinicopathological features,prognosis,immune cell infiltration,as well as diagnostic value was inspected by bioinformatics analysis using relevant R packages.The expression of ZIM2-AS1 in human normal liver cell line and HCC cell lines was examined by qRT-PCR.Results:The ex-pression of ZIM2-AS1 was highly expressed in HCC tissues(P<0.001),and its expression level was significantly correlated with age,gender,N stage,histologic grade and AFP level(P all<0.05).The overall survival(OS)and disease specific survival(DSS)of patients with high ZIM2-AS1 expression were significantly shorter than those of patients with low expression(P<0.05),and ZIM2-AS1 was an in-dependent risk factor affecting OS.Immune cell infiltration analysis showed that ZIM2-AS1 was closely related to the infiltration of Th2 cells,CD56brightNK cells,follicular helper T cells(Tfh),neutrophils and plasmacytoid dendritic cells(pDC)(|Spearman's r|>0.1,P<0.05)in HCC.ROC curve analysis revealed that the expression level of ZIM2-AS1 possesse potential diagnostic value in HCC,N0 stage,histologic grade G1 and G2,OS and DSS(AUC all>0.50).qRT-PCR results showed that the expression level of ZIM2-AS1 in HCC cell lines was significantly higher than that in human normal liver cells(P all<0.05).Conclusion:The elevated expression of lncRNA ZIM2-AS1 is an independent risk factor for poor prognosis of HCC patient and has potential application value as a biomarker for HCC diagnosis,prognosis as well as tumor immune microenvironment assessment.
