Tube-nesting EP:A new faster method to get more mouse bone marrow cells
10.3969/j.issn.1000-484X.2023.12.028
- VernacularTitle:嵌套EP管法更快速获取更多小鼠骨髓细胞
- Author:
Qiying XU
1
;
Zhuoya WANG
;
Yi YE
;
Tana WUREN
Author Information
1. 青海大学附属医院妇科,西宁 810001
- Keywords:
Tube-nesting EP;
Bone marrow cells;
Mouse
- From:
Chinese Journal of Immunology
2023;39(12):2624-2626,2630
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore a method to obtain more mouse bone marrow cells more quickly.Methods:Take both lower limbs of the same mouse,the bone marrow cells were obtained by either the tube-nesting EP method or the traditional syringe irrigation method,recorded the time and quantity of bone marrow cells,to analyze the proportion of viable cells by flow cytometry,and to detect the proliferation of these bone marrow cells used CCK-8 method.Results:Compared with the traditional syringe irrigation method,the time of obtaining mouse bone marrow cells by tube-nesting EP method was shorter(P<0.000 1).Compared with the traditional syringe irrigation method,the number of bone marrow cells obtained by tube-nesting EP method was more(P<0.05).The proportion of live cells and proliferation ability of bone marrow cells obtained by the two methods were identical.Conclusion:Tube-nesting EP method is a new faster method to get more mouse bone marrow cells.
