Role of N6-methyladenine regulators in prognosis and immune infiltration of cervical cancer
10.3969/j.issn.1000-484X.2023.12.022
- VernacularTitle:N6-甲基腺嘌呤调控因子对宫颈癌预后及免疫浸润的作用
- Author:
Yilin GUO
1
;
Yangyang BAI
;
Lu WANG
;
Zhen XU
;
Xiliang WANG
;
Wuliang WANG
Author Information
1. 郑州大学第二附属医院妇产科,郑州 450014
- Keywords:
N6-methyladenine;
Cervical cancer;
Prognosis;
Tumor microenvironment;
Immune infiltration
- From:
Chinese Journal of Immunology
2023;39(12):2588-2594
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore potential role and mechanism of N6-methyladenine(m6A)RNA methylation regulators in prognosis and tumor immune microenvironment of cervical cancer.Methods:Clinical and survival data as well as RNA sequencing data were acquired from TCGA and GEO database.Expressions and correlation among m6A RNA methylation regulators were analyzed by bioinformatics analysis and tissue validation experiments.LASSO Cox regression analysis was used to generate a prognostic risk sig-nature based on m6A regulators.Two m6A-modification patterns were identified based on m6A regulators and their abundance of im-mune cell inflitration were analyzed by ssGSEA.Results:Compared with normal tissues,RBM15,IGF2BP2,IGF2BP3,FMR1,YTHDF1,METTL3 and YTHDF2 expressions were significantly up-regulated in cervical cancer tissues,while ZC3H13,METTL4,YTHDC1,FTO and ALKBH3 expressions were significantly down-regulated.Five m6A regulators HNRNPC,LRPPRC,METTL3,ELAVL1 and FMR1 were selected to establish prognostic risk signature,whose expressions in cervical cancer were verified by HPA database and qRT-PCR with good predictive value,and could be used as independent poor prognostic factor(AUC=0.734).Based on expressions of 25 m6A regulators,two m6A modification patterns m6Acluster A and m6Acluster B were determined.There were differ-ences between two groups in biological functions,signal pathways and immune cells infiltration levels in tumor microenvironment.Conclusion:m6A RNA methylation regulators are closely related to prognosis and immune infiltration of cervical cancer,which provides guidance for immunotherapy strategy.
