Combined immunophenotyping to construct risk scoring model of lung adenocarcinoma ferroptosis-related lncRNA
10.3969/j.issn.1000-484X.2023.12.021
- VernacularTitle:联合免疫分型构建肺腺癌铁死亡相关长链非编码RNA风险评分模型
- Author:
Haiyang YU
1
;
Yueyin PAN
Author Information
1. 中国科学技术大学附属第一医院肿瘤化疗科,合肥 230001
- Keywords:
Lung adenocarcinoma;
lncRNA;
Ferroptosis;
Risk model
- From:
Chinese Journal of Immunology
2023;39(12):2582-2587
- CountryChina
- Language:Chinese
-
Abstract:
Objective:According lung adenocarcinoma(LUAD)ferroptosis and non-coding RNA in patients with a long-chain(lncRNA)correlation,binding immunophenotyping constructing new risk rating model to assess the prognosis of LUAD patients.Methods:Based on bioinformatics technology,download the transcriptome data and clinical data of LUAD samples from the TCGA database,obtain genes related to ferroptosis from the FreeDb database,and used"caret"package to screen 504 cases of LUAD samples and randomly divided into training set and validation set according to the ratios of 50%and 50%.Pearson correlation analysis and univariate-factor Cox regression were used to screen ferroptosis-related lncRNA related to the prognosis of LUAD,and the"Conen-susClusterPlus"package of R software was used.Immune correlation analysis with CIBERSORT software,LASSO regression analysis to establish ferroptosis-related lncRNA model,receiver operating characteristic(ROC)curve and area under the curve(AUC)to test the performance of the prognostic model,and verified by validation set.Results:Univariate factor Cox and LASSO regression analysis constructed nine risk scoring models composed of lncRNA related to ferroptosis.Both univariate and multivariate Cox regression analy-sis showed that this prognostic model can be used as an independent prognostic factor(P<0.001).The model had good prediction performance in training set,internal validation set and external validation set.Conclusion:The risk score model of LUAD patients constructed in this study can be used as a new independent prognostic evaluation method,or it may have further application value.
