Effect and mechanism of Asperosaponin Ⅵ on polarization of M1/M2 macrophages
10.3969/j.issn.1000-484X.2023.12.018
- VernacularTitle:川续断皂苷Ⅵ对M1/M2型巨噬细胞极化的调节作用及机制
- Author:
Jinfang LUO
1
;
Ming LIU
;
Hong YANG
;
Haibing QIAN
Author Information
1. 贵州中医药大学基础医学院药理学教研室,贵阳 550025
- Keywords:
Asperosaponin Ⅵ;
Lipopolysaccharide;
IL-4;
Polarization of macrophages
- From:
Chinese Journal of Immunology
2023;39(12):2566-2570,2576
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the regulation and mechanism of Asperosaponin Ⅵ on polarization of M1/M2 macrophages.Methods:MTT assay was used to detect the effects of Asperosaponin Ⅵ on RAW264.7 cell viability.The levels of TNF-α and IL-6 in supernatant of RAW264.7 cells induced by lipopolysaccharide(LPS)were determined by ELISA.The content of nitric oxide(NO)in supernatant of RAW264.7 cells induced by LPS was determined by Griess method.The gene expression levels of TNF-α,IL-6,argi-nase-1(Arg-1),heme oxygenase-1(HO-1)and suppressor of cytokine signaling protein-2(SOCS2)were detected by fluorescence quantitative PCR.Western blot was used to detect the expression levels of iNOS and p-p65 protein.Results:In LPS induced RAW264.7 cells,Asperosaponin Ⅵ inhibited protein or gene expression levels of TNF-α,IL-6,iNOS and p-p65,and increased HO-1 gene expression.Asperosaponin Ⅵ inhibited NO secretion in RAW264.7 cells induced by LPS.Asperosaponin Ⅵ increased the gene expression levels of M2 macrophage markers Arg1 and SOCS2 induced by IL-4.Conclusion:Asperosaponin Ⅵ inhibited RAW264.7 macrophage polarization to M1 type and promote it polarization to M2 type,which can play its anti-inflammatory and immunomodulato-ry role by regulating M1/M2 macrophage polarization.
