Influences of ginsenoside Rg1 on blood-brain barrier damage and neuroinflam-mation in rats with experimental cerebral hemorrhage by regulating miR-144-3p/FPR2/p38 signaling pathway
10.3969/j.issn.1000-484X.2023.12.013
- VernacularTitle:人参皂苷Rg1调节miR-144-3p/FPR2/p38信号通路对实验性脑出血大鼠血脑屏障损伤和神经炎症的影响
- Author:
Yalin BAI
1
;
Zhanhai FANG
;
Chenzhe DING
;
Yanping LAN
;
Dailin LIU
;
Gaoyang QI
;
Lei CHEN
;
Juncheng WANG
Author Information
1. 宁夏回族自治区人民医院,银川 740001
- Keywords:
Ginsenoside Rg1;
miR-144-3p;
Formyl peptide receptor 2/p38 pathway;
Experimental cerebral hemorrhage;
Neuroinflammation;
Blood-brain barrier
- From:
Chinese Journal of Immunology
2023;39(12):2534-2539
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate influences of ginsenoside Rg1 regulating miR-144-3p on neuroinflammation and blood-brain barrier damage in rats with experimental cerebral hemorrhage,and its regulation on formyl peptide receptor 2(FPR2)/p38 path-way.Methods:Ninety SD rats were randomly divided into control group,cerebral hemorrhage group,ginsenoside Rg1 low-dose group(10 mg/kg),ginsenoside Rg1 high-dose group(40 mg/kg),ginsenoside Rg1 high-dose+ago-miR-144-3p group(40 mg/kg ginseno-side Rg1+ago-miR-144-3p),with 18 mice in each group.Except for control group,experimental intracerebral hemorrhage rat model was constructed by injecting collagenase Ⅱ into right caudate nucleus,and then each group was given intraperitoneal administration and intracerebral injection as required.Neurological damage in rats was scored;rat brain water content was determined by dry-wet spe-cific gravity method;levels of TNF-α,IL-6 and IL-1β in rat brain tissues homogenate were determined by ELISA;ultrastructure around cerebral edema was observed by electron microscope;permeability of blood-brain barrier in rats was determined by Evans blue(EB)method;expressions of miR-144-3p/FPR2/p38 pathway were determined by qRT-PCR and Western blot.Results:Compared with control group,blood-brain barrier damage was aggravated in cerebral hemorrhage group,neurological function damage score,brain water content,miR-144-3p,TNF-α,IL-6,IL-1β,p38 mRNA,p-p38/p38 expressions in brain homogenate were increased(P<0.05),FPR2 mRNA and protein expressions were decreased(P<0.05);compared with cerebral hemorrhage group,blood-brain barrier damage was reduced in ginsenoside Rg1 low-dose group and ginsenoside Rg1 high-dose group,neurological function damage score,brain water content,miR-144-3p,TNF-α,IL-6,IL-1β,p38 mRNA,p-p38/p38 expressions in brain homogenate were decreased(P<0.05),FPR2 mRNA and protein expressions were increased(P<0.05);ago-miR-144-3p was able to reverse protective effects of gin-senoside Rg1 on blood-brain barrier and neuroinflammation in rats(P<0.05).Conclusion:Ginsenoside Rg1 may inhibit blood-brain barrier damage and neuroinflammation in rats by regulating miR-144-3p/FPR2/p38 axis.
