Curcumin inhibits oxidative stress in BCG-infected macrophages based on Nrf2 pathway
10.3969/j.issn.1000-484X.2023.12.011
- VernacularTitle:基于Nrf2通路姜黄素抑制卡介苗感染巨噬细胞氧化应激
- Author:
Jianqiu ZHAO
1
;
Xiaoqun HAN
;
Qin DENG
;
Jing YANG
;
Kuaiying WU
;
Huan HUANG
Author Information
1. 宜春学院,宜春 336000
- Keywords:
Curcumin;
Nrf2;
BCG;
Macrophages;
Oxidative stress
- From:
Chinese Journal of Immunology
2023;39(12):2523-2527
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of curcumin on oxidative stress in BCG-infected macrophages based on the Nrf2 pathway.Methods:THP-1-derived macrophages were infected.The experiment was divided into control group,BCG group,BCG+curcumin group and BCG+curcumin+ML385 group.Cellular ROS fluorescence intensity were observed under a fluores-cence microscope;Glutathione(GSH)levels were measured by Colorimetry;Western blot was used to detect the protein expressions of Nrf2,HO-1 and NQO1;MTT was used to detect the proliferation rate of macrophages.Results:BCG infection significantly enhanced ROS fluorescence intensity,reduced cell GSH content(P<0.01),inhibited protein expressions of Nrf2,HO-1 and NQO1,at the same time inhibited cell proliferation(P<0.01);curcumin significantly weakened ROS fluorescence intensity,increased GSH level(P<0.05),promoted Nrf2,HO-1 and NQO1 protein expressions and cell proliferation(P<0.01);Nrf2 inhibitor ML385 reversed the effect of curcumin.Conclusion:Curcumin can alleviate BCG-induced oxidative stress in macrophages by increasing the expression of Nrf2 and inducing the transcription of downstream antioxidant molecules.
