Neuroprotective effect of CDDO-Im on ischemic stroke in rats based on Nrf2/ARE signaling pathway
10.3969/j.issn.1000-484X.2023.12.009
- VernacularTitle:基于Nrf2/ARE通路探讨CDDO-Im对缺血性脑卒中大鼠的神经保护作用
- Author:
Xiaoxiao GUO
1
;
Mengke LIU
;
Huanhuan LIU
;
Jinggui SONG
Author Information
1. 新乡医学院第一附属医院神经内科,卫辉 453100
- Keywords:
Nrf2/ARE signaling pathway;
Ischemic stroke;
CDDO-Im;
Neuroprotection
- From:
Chinese Journal of Immunology
2023;39(12):2513-2516,2522
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the neuroprotective effect of synthetic triterpenoid(CDDO-Im)on ischemic stroke rats and its influence on inflammatory response by activating the nuclear factor 2-related factor 2(Nrf2)/antioxidant response elements(ARE)signaling pathway.Methods:SD rats were divided into sham group(S group),MCAO group(M group)and CDDO-Im inter-vention group(M+C group).The middle cerebral artery occlusion(MCAO)was used in M group and M+C group to establish ischemic stroke model in rats,and sham operation was used in S group to control.After operation,M+C group was injected with CDDO-Im(64 μg/300 g)every12 hours via caudal vein,S group and M group were given the same amount of normal saline.After 3 days,the nerve function of rats was measured by Longa score,and the area of cerebral infarction was evaluated by 2,3,5-triphenyltetrazolium chlo-ride(TTC)staining.The expressions of Nrf2,heme oxygenated-1(HO-1),ionic calcium binding adaptor molecule 1(Iba1),IL-1β and IL-4 protein were detected by Western blot.Results:Compared with S group,M group rats showed significant neurologic deficit and cerebral infarction area,and the expression of Nrf2 protein had no significant difference,and the expression levels of HO-1,Iba1,IL-1β and IL-4 protein were increased significantly(P<0.05).Compared with M group,the neurological deficit score,cerebral infarction area,the expression levels of Iba1 and IL-1β protein were decreased significantly(P<0.05),while Nrf2,HO-1 and IL-4 pro-tein expression increased significantly in M+C group(P<0.05).Conclusion:CDDO-Im may activate the Nrf2/ARE signaling pathway and play a neuroprotective role,which may be related to the modulation of microglia to M2 and the regulation of inflammatory response.
