Regulation of Th17/Treg immune imbalance by β-sitosterol in an OVA-induced allergic asthma rat model
10.3969/j.issn.1000-484X.2023.12.003
- VernacularTitle:β-谷甾醇对OVA诱导的过敏性哮喘大鼠模型中Th17/Treg免疫失衡的调节
- Author:
Jufang JIA
1
,
2
;
Mengnan ZENG
;
Beibei ZHANG
;
Ru WANG
;
Meng LIU
;
Pengli GUO
;
Qinqin ZHANG
;
Fengyu ZHANG
;
Xiaoke ZHENG
;
Weisheng FENG
Author Information
1. 河南中医药大学药学院,郑州 450046
2. 河南省中药开发工程技术研究中心,郑州 450046
- Keywords:
β-sitosterol;
Allergic asthma;
OVA;
Th17/Treg immune imbalance
- From:
Chinese Journal of Immunology
2023;39(12):2477-2482
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the interventional effect of β-sitosterol on ovalbumin(OVA)-induced allergic asthma rats and its potential mechanism.Methods:SD male rats were randomly divided into normal group(CON),model group(M),positive drug dexamethasone group(DEX,0.075 mg/kg)and β-sitosterol group(Sit,50 mg/kg).A rat model of allergic asthma was estab-lished by intraperitoneal injection of OVA with aluminum hydrogen solution,and nebulized inhalation of OVA to stimulate.Rats were given intragastric administration 30 min before aerosol challenge,and after continuous administration for 7 days,the indicators of cough and asthma and tracheal phenol red excretion were detected.HE staining was used to observe pathological changes of lung tis-sue.Flow cytometry was used to detect reactive oxygen species(ROS)generation,apoptosis level and ratios of Th17 and Treg cells in peripheral blood.Biochemical method was used to detect contents of MDA,and activities of T-SOD and GSH-Px in rat lung tissues.ELISA was used to detect levels of Th17 and Treg-related cytokines(TNF-α,IL-4,IL-6,IL-17A,and IL-35).Results:Compared with model group,β-sitosterol significantly prolonged the incubation period of cough and gasp in rats with allergic asthma,reduced the frequency of cough and gasping,and promoted the excretion of phenol red in trachea;significantly reduced inflammatory infiltration in lung tissue of asthmatic rats;observably reduced MDA content in lung tissue,ROS of primary lung cell and apoptosis levels of asthmatic rats,increased the activities of T-SOD and GSH-Px;markedly reduced proportion of Th17 cells and levels of pro-inflammatory cyto-kines TNF-α,IL-4,IL-6 and IL-17A,increased proportion of Treg cells and levels of anti-inflammatory cytokine IL-35.Conclusion:β-sitosterol can ameliorate airway inflammation and oxidative damage in OVA-induced allergic asthmatic rats,and its mecha-nism may be related to the regulation of β-sitosterol on Th17/Treg immune imbalance and oxidative stress response.
