Metabonomic study of blood of mice with high-voltage electrical injury
- VernacularTitle:高压电击伤小鼠的血液代谢组学研究
- Author:
Si-Yu CHEN
1
;
Hui WANG
;
Yan LUO
;
Jia-Wen TAO
;
Wen-Juan ZHANG
;
Yang YUE
;
Zheng-Ping YU
;
Hui-Feng PI
Author Information
- Keywords: electrician operation; high-voltage electrical injury; blood; blood biochemistry; metabolomics
- From: Journal of Regional Anatomy and Operative Surgery 2024;33(2):100-106
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the changes of metabonomics in blood of mice after high-voltage electric shock,then screen out the significantly changed differential metabolites and metabolic pathways.Methods The head of C57BL/6J mice was subjected to high-voltage electric shock(electric shock group)or exposed to acoustic and optical stimulation of high-voltage electric(control group),then the whole blood from mice were collected to separate serum.The dual platform combined metabonomic analysis based on gas chromatography-mass spectrometer(GC-MS)and liquid chromatography-mass spectrometer(LC-MS)was performed and orthogonal partial least squares discriminant analysis(OPLS-DA)was used to screen the differential metabolites and related metabolic pathways.Results A total of 415 differential metabolites were screened out in metabonomics in blood of mice after high-voltage electric shock,including 187 up-regulated and 228 down-regulated metabolites.These differentially metabolites were significantly enriched in metabolic pathways including central carbon metabolism in cancer,glucagon signaling pathway,etc.Conclusion By establishing the model of high-voltage electrical injury on experimental mice,this study reveals the significant change of metabolite content and metabolic pathway in blood by high-voltage electrical injury.Which provides a basis for the damage of blood metabolic activity by high-voltage electrical injury,and suggests the potential harm of high-voltage electrical injury to blood metabolic activity in the whole body.
