Ten kinds of antipyretic-antidotal traditional Chinese medicine extracts against extensively drug-resistant Acinetobacter baumannii infection
10.12138/j.issn.1671-9638.20244696
- VernacularTitle:十种清热解毒中药提取物抗泛耐药鲍曼不动杆菌感染的研究
- Author:
Yan YANG
1
,
2
;
Jian-Wen FENG
;
Bo JI
;
Jin YUAN
;
Yan WANG
;
Jian LI
;
Xue-Jiu CAI
;
Zhi-Hui JIANG
Author Information
1. 南方医科大学药学院,广东广州 510515
2. 中国人民解放军南部战区总医院临床药学科,广东广州 510010
- Keywords:
antipyretic-antidotal TCM;
extensively drug-resistant Acinetobacter baumannii;
Caenorhabditis ele-gans;
infection model;
traditional Chinese medicine
- From:
Chinese Journal of Infection Control
2024;23(3):271-276
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the activity of ten kinds of antipyretic-antidotal traditional Chinese medicine(TCM),including radix tinosporae.herb of blin conyza and turmeric,against extensively drug-resistant Acineto-bacter baumannii(XDR-AB)infection,screen out the extracts of antipyretic-antidotal TCM which have in vivo anti-infection activity,provide a research basis for the discovery of novel antimicrobials against XD-RAB infection.Methods Ten antipyretic-antidotal TCM were extracted with water,50％ethanol and 95％ethanol respectively,and TCM extracts with different concentrations were prepared,which were co-incubated with the model of XDR-AB-infected Caenorhabditis elegans previously optimized by the research group.The in vivo activity of antipyretic-antidotal TCM against XDR-AB infection was judged through the survival rate of Caenorhabditis elegans.Results With the increase of concentration of turmeric and cortex pseudolaricis extracts,the survival rate of XDR-AB-infec-ted nematodes continued to improve.The water extract,50％ethanol extract,and 95％ethanol extract of turmeric at a concentration of 1 000 μg/mL could increase the survival rates of XDR-AB-infected Caenorhabditis elegans to 54.2％(compared to the negative control group,P＜0.001),18.8％,and 13.3％,respectively.The water ex-tract,50％ethanol extract,and 95％ethanol extract of cortex pseudolaricis at a concentration of 1 000 μg/mL could increase the survival rates of XDR-AB-infected Caenorhabditis elegans to 47.4％(compared to the negative control group,P＜0.001),23.8％,and 15.8％,respectively.Conclusion The water extracts of turmeric and cortex pseudolaricis have good activity against XDR-AB infection,and their main chemical components can be tested for in vitro antimicrobial efficacy to discover novel antimicrobial agents against XDR-AB infection.
