Evaluating the application efficiency of the MHSeqTyper47 kit in kinship identification
10.13618/j.issn.1001-5728.2024.01.013
- VernacularTitle:MHSeqTyper47试剂盒在亲缘关系鉴定中的应用效能评估
- Author:
Lishuai TAN
1
,
2
;
Xin JIN
;
Yaosen FENG
;
Kelai KANG
;
Wenhua MA
;
Mingguang LI
;
Chi ZHANG
;
Jie ZHAO
;
Jian YE
;
Le WANG
Author Information
1. 山西医科大学法医学院,山西晋中 030600
2. 法医遗传学公安部重点实验室,公安部鉴定中心,北京 100038
- Keywords:
Forensic genetics;
Microhaplotype;
Kinship identification;
MHSeqTyper47 kit;
ITO method
- From:
Chinese Journal of Forensic Medicine
2024;39(1):75-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application value of the MHSeqTyper47 kit in kinship identification.Methods Multiplexed amplification and library preparation were performed for DNA samples from 113 related individuals by using the MHSeqTyper47 kit.The libraries were sequenced on a MiSeq FGx sequencer,and the data were analyzed using MHTyper for microhaplotype genotyping.The kinship indexes were calculated to evaluate the application efficiency of this kit in kinship identification and compared with those of the GlobalFilerTM kit.Results For the MHSeqTyper47 kit,the CPI values in trio identification were 1.43× 1011～6.15×1018.The CPI values in duo identification were 1.02× 105～1.53× 1013.The CFSI values in full sibling identification were 7.73×101～2.59×1016.Trios,duos and full siblings could be completely distinguished from unrelated pairs.The combined efficiency of these two kits in 2nd-degree kinship identification was 0.466 2.Conclusion The application value of MHSeqTyper47 kit is relatively higher in the identification of lst-degree kinships.If jointly used with the GlobalFilerrM kit,2nd-degree kinship identification could be achieved in some cases.
