Determination of hydrogen sulfide in Blood by LC-MS/MS
10.13618/j.issn.1001-5728.2024.01.009
- VernacularTitle:血液中硫化氢的LC-MS/MS法检验
- Author:
Xiaojun WU
1
;
Ge QIN
;
Chunming WEI
;
Peng ZHAO
;
Jiayi LI
;
Jing CHANG
;
Yunfeng ZHANG
Author Information
1. 公安部鉴定中心,北京 100038
- Keywords:
Blood;
H2S;
LC-MS/MS;
Gaseous poison;
Forensic toxicology
- From:
Chinese Journal of Forensic Medicine
2024;39(1):55-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining hydrogen sulfide(H2S)in blood and apply it to practical cases.Methods A delute solution was achieved by adding 0.8 mL saturated borax solution into 0.2 mL blood sample was diluted with.1 mL acetonitrile solution containing 0.1％formic acid was then taken in a test tube,followed by adding 0.1 mL dilute solution and 0.1 mL thiozine aqueous solution(1％).After thorough mixing,the mixture was left to stand for 30 minutes.Subsequently,the sample was subjected to liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis after centrifugation and membrane filtration.Results The results showed that H2S exhibited good linearity within the concentration range of 10～2 000 ng/mL,with the R2 value of 0.998 5.The detection limit was 5 ng/mL,and the quantification limit was 10 ng/mL.In three cases of H2S poisoning,sulfur ions were detected in the blood of the deceased individuals,with concentrations ranging from 0.17 to 0.56 μg/mL.Conclusion For the first time,this study established a LC-MS/MS method for determining H2S in blood,which can meet the detection needs of H2S poisoning cases.
