Effects of l-tetrahydropalmatine on different stages of conditioned place preference in ketamine dependent rats and the mechanism of DA
10.13618/j.issn.1001-5728.2024.01.008
- VernacularTitle:l-THP对氯胺酮依赖大鼠不同阶段条件位置偏爱的影响及DA作用机制研究
- Author:
Yan DU
1
,
2
;
Xingcui GAO
;
Hongliang SU
;
Yujin WANG
;
Li DU
;
Keming YUN
Author Information
1. 中国辐射防护研究院,山西太原 030006
2. 山西医科大学药学院,山西太原 030001
- Keywords:
Levo-tetrahydropalmatine;
Ketamine;
Conditioned place preference;
Dopamine;
UPLC-MS/MS
- From:
Chinese Journal of Forensic Medicine
2024;39(1):50-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Levo-tetrahydropalmatine(l-THP)on the expression,regression and relapse of conditioned place preference(CPP)in ketamine induced rats,and to detect the content of dopamine(DA)in the striatum(caudate putamen,CPu)of the rat brain at different time points.Methods Ketamine addiction rat model was established by CPP.The effects of l-THP on the expression,regression and relapse of ketamine induced rat CPP were investigated using CPP score as the index.The content of DA in CPu of rats was determined by ultra-performance liquid chromatography coupled to tandem mass spectrometry(UPLC-MS/MS)after ketamine administration and l-THP intervention at 30 min,60 min,90 min,120 min and 150 min.Results It indicated that 1-THP could decrease the expression of CPP in ketamine induced rats,promote the process of CPP resolution and inhibit the process of relapse.In addition,l-THP combined with ketamine administration significantly inhibited the ketamine-induced increase in DA content in the CPu of the rats.Conclusion The mechanism of l-THP inhibiting the reward effect of ketamine may be related to blocking DA receptors and reducing the release of DA neurotransmitters.l-THP has potential implications for the treatment of ketamine addiction.
