Study on enzymatic hydrolysis conditions of cannabis,MDMB-4en-PINACA and ADB-BUTINACA in urine
10.13618/j.issn.1001-5728.2024.01.007
- VernacularTitle:尿液中大麻及合成大麻素MDMB-4en-PINACA、ADB-BUTINACA酶水解条件研究
- Author:
Jiali ZHANG
1
,
2
;
Xin WANG
;
Ping XIANG
Author Information
1. 山西医科大学法医学院法医学山西省重点实验室,山西晋中 030600
2. 司法鉴定科学研究院上海市法医学重点实验室上海市司法鉴定专业技术服务平台司法部司法鉴定重点实验室,上海 200063
- Keywords:
Forensic toxicological analysis;
Enzymatic hydrolysis;
Synthetic cannabinoids(SCs);
MDMB-4en-PINACA;
ADB-BUTINACA;
Δ9-THC-COOH
- From:
Chinese Journal of Forensic Medicine
2024;39(1):44-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective Synthetic cannabinoids(SCs)will be widely metabolized to phase I metabolites and glucuronide metabolites after entering human body.It is usually difficult to detect the parent drug of SCs in urine.Therefore,it is necessary to undergo de-glucuronidation in the preparation of urine samples.We aimed to optimize the enzymatic hydrolysis methods for SCs detection in urine.Methods We studied enzymatic hydrolysis for the determination of 11-demethyl-9-carboxyl-THC(Δ9-THC-COOH)in THC positive urine samples by liquid chromatography-tandem mass spectrometry(LC-MS/MS),and compared it with the alkaline digestion method.Meanwhile,we studied enzymatic hydrolysis for the determination of the related metabolites in MDMB-4en-PINACA and ADB-BUTINACA positive urine samples.Results The Δ9-THC-COOH glucuronic acid conjugate could be hydrolyzed by adding 3μL β-d-glucuronidase solution(＞100 000 units/mL)for 30 min at 55℃.The MDMB-4en-PINACA M and ADB-BUTINACA M glucuronide conjugates were hydrolyzed by adding 3μL β-d-glucuronidase solution(＞100 000 units/mL)for 30 min at 75℃.Conclusion This study can provide reference for the establishment of rapid,accurate and reliable enzymatic hydrolysis methods when detecting SCs in urine.
