Study on metabolic changes of myocardial tissue in rats exposed to macleaya cordata
10.13618/j.issn.1001-5728.2024.01.006
- VernacularTitle:博落回中毒大鼠心肌组织代谢产物变化的研究
- Author:
Shiyong FANG
1
;
Xiaoling SHI
;
Jing ZHANG
;
Yukun LU
;
Pei FANG
;
Guang CHEN
;
Maowang WU
;
Xiang XU
Author Information
1. 皖南医学院法医学院,安徽芜湖 241000
- Keywords:
Forensic toxicology;
Toxicity;
Metabolomics;
Macleaya cordata;
Gas chromatography-mass spectrometry
- From:
Chinese Journal of Forensic Medicine
2024;39(1):39-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the metabolic changes of myocardial tissue in rats under acute exposure to macleaya cordata by gas chromatography mass spectrometry(GC-MS),explore forensic identifications of its characteristic metabolites,and verify its toxicological mechanism in poisoning cases.Methods The rats in the exposure group were given 382 mg/kg macleaya extract solution by gavage,and the rats in the control group were given the same dose of solvent.The myocardial samples were analyzed by GC-MS,and pattern recognition was conducted through partial least squares discriminant analysis(PLSDA).The differential metabolites with characteristic changes were identified by variable importance projection(VIP value＞1)and Student's t test(P＜0.01).Results Compared with the control group,21 potential characteristic metabolites were identified.Through KEGG pathway enrichment analysis,it was found that these metabolites were mainly involved in the pathways of glycine,serine and threonine metabolism;pyruvate metabolism and glycerolipid metabolism.Conclusion Through the study of myocardial metabolism in rats exposed to macleaya cordata,we found the information on metabolites closely related to poisoning,which provides new insight and reference for studies on the mechanisms of macleaya cordata poisoning in the field of forensic medicine.
