Identification of 15 bird remains using the 16S rRNA gene sequence
10.13618/j.issn.1001-5728.2023.06.019
- VernacularTitle:应用16S rRNA基因序列鉴定15只鸟类残体
- Author:
Hong ZHU
1
,
2
;
Jianshen CAO
;
Suwei SHANG
;
Liu YANG
;
Hepeng LI
Author Information
1. 浙江省林业科学研究院,浙江 杭州 310023
2. 浙江省湿地研究中心,浙江 杭州 310023
- Keywords:
Bird remains;
DNA barcode;
16S rRNA;
Molecular identification
- From:
Chinese Journal of Forensic Medicine
2023;38(6):702-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective The mitochondrial 16S rRNA gene PCR sequencing method was applied to identify the bird species involved in the case of bird remains.Methods Using frozen muscle tissue samples from 15 unknown bird remains,the PCR amplification of the 16S rRNA DNA barcode fragment was performed.Results Through sequence alignment and phylogenetic analysis,it was determined that the 15 samples were associated with six bird species,including four Streptopelia chinensis,one Turdus eunomus,five Passer montanus,two Chloris sinica,two Fringilla montifringilla,and one Phoenicurus auroreus.These species belong to 2 orders,6 families,and 6 genera,all of which are protected as listed species under the wildlife conservation regulations.Conclusion The 16S rRNA gene segment can be regarded as a reliable approach for accurately identifying bird species from remains,providing a dependable basis for qualitative and sentencing determinations in judicial cases.
