UPLC-HRMS determining p-methoxymethamphetamine and its metabolites in rabbit blood
10.13618/j.issn.1001-5728.2023.06.018
- VernacularTitle:UPLC-HRMS分析家兔血液中对甲氧基甲基苯丙胺及其代谢产物
- Author:
Chao YUAN
1
;
Yunfeng ZHANG
;
Fengqin FAN
;
Guisong LIU
;
Rui NIU
Author Information
1. 日照市公安局,山东 日照,276800
- Keywords:
High-performance liquid chromatography-hrms;
P-methoxymethamphetamine;
Metabolites
- From:
Chinese Journal of Forensic Medicine
2023;38(6):699-701
- CountryChina
- Language:Chinese
-
Abstract:
The high-performance liquid hromatography-quadrupole-electrostatic field orbital well high-resolution mass spectrometry method was developed for the analysis of p-methoxymethamphetamine(PMMA)and its metabolites in rabbit blood.Hypersil Gold aQ(2.1 mm×100 mm,1.9 μm)was used as the chromatographic column.The mobile phase was gradient elution with 0.1%formic acid aqueous solution(A)and 0.1%formic acid acetonitrile(B)at a flow rate of 0.3 ml/min.Electrospray ionization(ESI)with positive and negative mode scanning was used to determine p-methoxymethamphetamine and its metabolites based on excimer and secondary fragment ions.PMMA and its metabolites were characterized by high-performance liquid chromatography-high resolution mass spectrometry,which lays a foundation for the study of its pharmacodynamic substance and prevention.
