Mutation characteristics of rpoB gene in rifampicin-resistant Brucella strains
10.3760/cma.j.cn231583-20221102-00363
- VernacularTitle:布鲁氏菌利福平耐药株rpoB基因突变特征分析
- Author:
Ying ZHENG
1
;
Xiaojing MA
;
Liya LIU
;
Feng YE
;
Wenxi GU
;
Xinping YI
Author Information
1. 新疆农业大学动物医学学院，乌鲁木齐 830052
- Keywords:
Brucella;
Rifampicin;
Resistance;
rpoB gene
- From:
Chinese Journal of Endemiology
2024;43(2):94-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the mutation characteristics of rpoB gene in rifampicin-resistant Brucella strains. Methods:DNA of 4 rifampicin-resistant Brucella strains (JSY-26, G-9, WSY-13 and AW-3) isolated from Xinjiang Uygur Autonomous Region was selected, rifampicin rpoB gene was amplified by PCR and its nucleotide sequence was sequenced. The rpoB gene sequences of rifampicin-resistant Brucella standard strain (RB51) and sensitive strain (ALT-8) were used as reference, the mutation sites and types of the rpoB gene inside and outside the rifampicin resistance determination region (RRDR) of the 4 rifampicin-resistant Brucella strains were analyzed by Mega 7.0 software. Results:Through sequence alignment, both JSY-26 and WSY-13 strains underwent a single base point mutation at the RRDR 1 576 bp of the rpoB gene, with the base changing from guanine (G) to adenine (A). The G-9 strain underwent a single base point mutation at the RRDR 1 606 bp of the rpoB gene, with the base changing from cytosine (C) to A. The AW-3 strain showed 5 mutations of 3 types outside rpoB gene RRDR at 2 536, 2 537, 2 626, 2 636 and 2 654 bp, namely 3 insertion mutations [thymine (T) insertion once and C insertion twice], 1 deletion mutation (C deletion), and 1 single base point mutation (from G to C mutation).Conclusion:The RRDR mutations in the rpoB gene of the rifampicin-resistant Brucella strains are mainly characterized by single base point mutations, while multiple insertion and deletion mutations occur outside the RRDR.
